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EpCAM Antibody

CAT: 0013-GTR18573899Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18573899Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]
UniProt
P16422
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the EpCAM antibody.
Clonality
Monoclonal
Clone
EPM17-1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/million cells in 0.1ml, Western blot: 2-4ug/ml, Immunofluorescence: 1-2 ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Titering of the EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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