TGFB1 ELISA Kit (Human)

The coefficient of variation of both intra-assay and inter-assay were less than 10%.

7732-18-5

Leptin

OB, OBS, LEPD

3952

Human

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.

ELISA Kit

ELISA

Colorimetric, OD450 nm

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Cell culture, supernates, serum, and plasma

15 pg/ml

Human

Components 2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human Leptin antibody 2 vials Streptavidin-HRP solution 4 bottles Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 vials human MCP-1 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human MCP-1 monoclonal antibody Components Components 2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human Leptin antibody 2 vials concentrated Biotin-Conjugate anti-human Leptin antibody 2 vials Streptavidin-HRP solution 2 vials Streptavidin-HRP solution 4 bottles Standard /sample Diluent 4 bottles Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Substrate Solution 1 vial Stop Solution 1 vial Stop Solution 2 vials human MCP-1 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials human MCP-1 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human MCP-1 monoclonal antibody 2 vials concentrated Biotin-Conjugate anti-human MCP-1 monoclonal antibody

LEP

LEP