TGFB1 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 114 240 483 125 248 515 St. Dev. 5.47 12 17.9 6.75 15.4 30.4 %CV 4.79 5 3.70 5.4 6.20 5.90 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 114 240 483 125 248 515 Mean (pg/mL) 114 240 483 125 248 515 St. Dev. 5.47 12 17.9 6.75 15.4 30.4 St. Dev. 5.47 12 17.9 6.75 15.4 30.4 %CV 4.79 5 3.70 5.4 6.20 5.90 %CV 4.79 5 3.70 5.4 6.20 5.90

• Gene Name:

  Transforming growth factor, beta 1

• Gene Aliases:

  Camurati-Engelmann disease, CED, DPD1, LAP, Latency-associated peptide, TGF beta 1 protein, TGF-beta-1, TGFB, tgfb1, TGFB1_HUMAN, TGFbeta, Transforming growth factor beta 1

• Gene ID:

  21803

• Accession Number:

  NP_035707.1

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: CHO; Immunogen sequence: A279-S390

• Target:

  Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.The standard product used in this kit is recombinant TGFβ1 with the molecular mass of 25KDa.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF beta 1 has been precoated onto 96-well plates. Standards (CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TGF beta1 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum, plasma (EDTA) and urine

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <2 pg/ml

• Homology:

  Cross-reactivates with TGFB2, TGFB3, TGFB5 <1%.

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Notes:

  Formerly GWB-ZZD047_x005F

• Specificity:

  Natural and recombinant mouse TGF beta1

• NCBI Gene Symbol:

  TGFB1

• Protein Name:

  Transforming growth factor beta-1

• Gene Name URL:

  TGFB1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_011577.2