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Lipoxin A4 ELISA Kit

CAT: 0026-E4788-100Size: 96 AssaysDry Ice: NoHazardous: No
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CAT#:0026-E4788-100Size:96 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Lipoxin A4 (LXA4), an endogenous lipoxygenase-derived eicosanoid mediator, has potent dual pro-resolving and anti-inflammatory properties. Endogenous transcellular biosynthesis of LXA4 occurs via interaction of leukocytes with epithelium, endothelium or platelets. Lipoxins (LXs) or the lipoxygenase interaction products are generated from arachidonic acid via sequential actions of lipoxygenases and subsequent reactions to give specific trihydroxytetraene-containing eicosanoids. BioVision Lipoxin A4 ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Lipoxin A4. During the reaction, Lipoxin A4 in the samples or standard competes with Lipoxin A4 coated on the plate for binding to the anti-Lipoxin A4 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Lipoxin A4. The concentration of Lipoxin A4 in the samples can be calculated by comparing the OD of the samples to the standard curve
CAS Number
7732-18-5
Reactivity
Universal
Applications
in vitro quantitative measurement of Lipoxin A4 in rat serum, plasma and other biological samples.
Detection Method
Absorbance (450 nm)
Features & Benefits
Specificity: This kit recognizes LXA4 in samples. No Significant cross-reactivity or interference between LXA4 and analogues was observed., Sensitivity: 0.47 ng/mL, Precision: Coefficient of variation is < 10%., Detection Range: 0.78-50 ng/mL
Shipping Conditions
Gel Pack
Storage Conditions
An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
Shelf Life
12 months

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