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TNF Antibody

CAT: 0013-GTR18187507Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187507Size:25 µL
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Description
TNF antibody
Product Name Alternative
APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
UniProt
P01375
Reactivity
Human, Primate
Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant human TNFa produced in E.coli.
Target
TNF
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
75 mg/mL
Dilution
ELISA: 1:1,000 - 1:5,000, IHC: 1:100 - 1:500, IF: 1:100, WB: 1:500 - 1:2,000
Purity
This antiserum has been heated to 56°C for 30 minutes. The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin) . This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Human TNFα has been tested for use in immunohistochemistry, immunofluorescence, and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
P01375.1
Host or Source
Rabbit
Preservative
None. None
Isotype
Antiserum

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