POLR2A Antibody

CAT: 0013-GTR18187214Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18187214Size:25 µL

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Description

POLR2A antibody

Product Name Alternative

RPO2, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RPB1, POLR2A

UniProt

P24928

Reactivity

Human

Immunogen

Anti-POL II antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminal portion of human POL II conjugated to Keyhole Limpet Hemocyanin (KLH) .

Target

POLR2A

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1.0 mg/ml

Dilution

ELISA: 1:10,000 - 1:50,000, WB: 1:1000

Purity

This affinity purified antibody is directed against human POL II. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with mouse and Chinese hamster.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-POL II antibody has been tested in ChIP and ELISA, and is likely suitable for western blotting and immunofluorescence assays. Positive controls used in ChIP were EGF stimulated NR4A1 5', NR4A1 3' and FOS 3'. The negative control was total rabbit IgG.

Tested Applications

ChIP, ELISA, WB

NCBI Accession Number

NP_000928.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P24928

DNA-directed RNA polymerase II subunit RPB1

RPB1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P24928
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: POLR2A
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: RNA polymerase II subunit B1
EC number: 2.7.7.6
Processing: —
Secondary accessions: A0AAG2TJB2, A6NN93, B9EH88, Q6NX41

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondMethylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

ChromosomeCytoplasmDNA-directed RNA polymeraseNucleus

Disease

Disease variantIntellectual disability

Molecular function

DNA-bindingHydrolaseNucleotidyltransferaseRNA-directed RNA polymeraseTransferase

Biological process

Host-virus interactionTranscription

Ligand

MagnesiumMetal-bindingZinc

Domain

Repeat

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