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PAX6 Antibody

CAT: 0013-GTR18186934Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18186934Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PAX6 antibody
Product Name Alternative
Mouse anti-pax6 antibody, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, AN2, PAX 6 Antibody
UniProt
P26367
Reactivity
Human
Immunogen
PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein.
Target
PAX6
Clonality
Monoclonal
Clone
7H10.D3.D4.G5
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:75,000 - 1:150,000, IF: 1:100, WB: 1:500 - 1:2,000
Purity
Anti-PAX6 was purified from clarified ascites by Protein A chromatography. Cross-reactivity with PAX6 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-PAX6 antibody has been tested by ELISA and Immunofluorescence and suitable for Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46.7 kDa in size corresponding to PAX6 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, WB
NCBI Accession Number
NP_000271.1
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG1

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