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LacI Antibody

CAT: 0013-GTR18186775Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186775Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Lac I antibody
Product Name Alternative
Rabbit anti-LacI antibody, rabbit anti-Lac-I antibody, Rabbit anti-Lac I antibody, Lactose operon repressor, DNA binding transcriptional repressor antibody, Transcriptional repressor of the lac operon antibody
UniProt
P03023
Reactivity
Bacteria, E. coli
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.
Target
LacI
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:90,000 – 1:100,000, WB: 1:500 - 1:2,000
Purity
This Anti-Lac I product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Rabbit anti-Lac-I antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kDa in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
146576
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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