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ABCB5 Antibody

CAT: 0013-GTR18186760Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18186760Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ABCB5 antibody
Product Name Alternative
Rabbit anti-ABCB5 Antibody, ABCB 5, ABCB-5, ATP binding cassette sub family B (MDR/TAP) member 5 antibody, ATP binding cassette sub family B member 5 antibody, EST422562 antibody, P glycoprotein ABCB5 antibody
UniProt
Q2M3G0
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
Target
ABCB5
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
0.31 mg/mL
Dilution
ELISA: 1:10,000 - 1:15,000, IHC: 1:200, WB: 1:10,000
Purity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ABCB5 protein. A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources. Cross-reactivity with ABCB5 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, IHC, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 117 kDa in size corresponding to ABCB5 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
56849536
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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