GSK3B Antibody

CAT: 0013-GTR18186592Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18186592Size:100 µg

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Description

GSK3 Beta (phospho-S9) antibody

Product Name Alternative

Rabbit anti-GSK3 beta pS9 antibody, rabbit anti-GSK3 ß pS9 antibody, Glycogen Synthase Kinase-3 Beta antibody, GSK-3 beta antibody, GSK 3B antibody, GSK-3B antibody, GSK3B protein antibody, GSK3beta isoform antibody, Serine/threonine-protein kinase GSK3B, phosphorylated GSK3ß

UniProt

P49841

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near aa 1-15 of human GSK3 beta.

Target

GSK3B

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IF: 1:200 - 1:1,000, WB: 1:500 - 1:3,000

Purity

This affinity purified antibody is directed against the phosphorylated form of human GSK3B at the pS9 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, rat, frog, chicken, dog and zebrafish based on 100% homology for the immunogen sequence. Cross reactivity with GSK3B homologues from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This antibody has been tested for use in ELISA, immunohistochemistry, western blotting and immunoprecipitation. Reactivity in other immunoassays is unknown. Serum starved 293T whole cell lysate is suitable for use as a positive control. Anti-GSK 3B shows a strong signal to GSK 3B in westerns at the estimated molecular weight of 47 kD. This antibody may show very weak reactivity against GSK 3A.

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

21361340

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P49841

Glycogen synthase kinase-3 beta

GSK3B_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P49841
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: GSK3B
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: GSK-3 beta
EC number: 2.7.11.26
Processing: —
Secondary accessions: D3DN89, Q9BWH3, Q9UL47

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

ADP-ribosylationLipoproteinPalmitatePhosphoprotein

Coding sequence diversity

Alternative splicing

Disease

Alzheimer diseaseDiabetes mellitus

Ligand

ATP-bindingNucleotide-binding

Biological process

Biological rhythmsCarbohydrate metabolismDifferentiationGlycogen metabolismNeurogenesisWnt signaling pathway

Cellular component

Cell membraneCytoplasmMembraneNucleus

Molecular function

Developmental proteinKinaseSerine/threonine-protein kinaseSignal transduction inhibitorTransferase

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