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AP2A Antibody

CAT: 0013-GTR18186559Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186559Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AP2A antibody
Product Name Alternative
Goat anti-AP2A Antibody, AP-2 complex subunit alpha-1, Adapter-related protein complex 2 alpha-1 subunit, Adaptor protein complex AP-2 subunit alpha-1 Alpha1-adaptin, Plasma membrane adaptor HA2/AP2, Clathrin assembly protein complex 2 alpha-A large chain, AP2A2, ADTAB, CLAPA2, HIP9, HYPJ, KIAA0899
UniProt
O95782
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of Human AP2A1 and AP2A2 proteins.
Target
AP2A
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.7 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
Purity
This affinity purified antibody is directed against human AP2A. The product was affinity purified from antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with AP2A1 and AP2A2 protein from human, chimpanzee, orangutan, mouse, rat, chicken and dog based on 100% homology for the immunogen sequence. Cross reactivity with AP2A proteins from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 104-107 kDa in size corresponding to AP2A1 or AP2A2 proteins by western blotting in the appropriate cell lysate or extract. Multiple isoforms of each subunit exist for these proteins. The immunogen sequence is found in both AP2A1 and AP2A2. This antibody recognizes a band of approximately 100 kDa in multiple human cell lines and specific reactivity can be blocked with the immunizing peptide.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
27477041
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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