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Phosphothreonine Antibody

CAT: 0013-GTR18186553Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18186553Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Phosphothreonine antibody
Product Name Alternative
Mouse anti-Phosphothreonine Antibody, mouse anti-pT antibody
Reactivity
Other
Immunogen
Phosphothreonine antibody was produced after repeated immunizations of balb/c mice with phosphothreonine conjugated KLH.
Clonality
Monoclonal
Clone
18F6
Conjugation
Unconjugated
Dilution
ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
Purity
This product is clarified ascites produced in balb/c mice using clone 18F6. Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This monoclonal antibody has been tested in ELISA and Western Blot and reacts specifically with phosphothreonine and shows minimal reactivity by ELISA, and competitive ELISA, with phosphoserine or phosphotyrosine. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, as well as phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases and is important for cell signaling in activation, proliferation and differentiation.
Tested Applications
ELISA, WB
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. None
Isotype
ASCITES

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