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TNF Antibody

CAT: 0013-GTR18186551Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18186551Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TNF Alpha antibody
Product Name Alternative
Rabbit anti-TNF-A antibody, rabbit anti-TNFα antibody, rabbit anti-TNF alpha antibody, cachexin, cachectin, tumor necrosis factor-alpha, TNFSF2, TNFSF1A
UniProt
P23563
Reactivity
Porcine
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFα.
Target
TNF
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
1.15 mg/mL
Dilution
ELISA: 1:2,000 - 1:2,500, WB: 1:1000
Purity
This product was Protein-A purified from monospecific antiserum by chromatography. It is specific for swine TNFα protein. A BLAST analysis was used to suggest cross-reactivity with TNFα from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFα from baboon based on 90% homology. Cross-reactivity with TNFα from sources other than swine has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This protein A purified TNFα antibody has been tested by western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to swine TNFα by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_999187
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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