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SMT3 Antibody

CAT: 0013-GTR18186979Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186979Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SUMO antibody
Product Name Alternative
Rabbit anti-SUMO Antibody, Ubiquitin-like protein SMT3 antibody, SMT3 antibody, Ubiquitin like protein of the SUMO family antibody, SMT3_YEAST antibody, DmSUMO 1 antibody
UniProt
Q12306
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast SUMO protein.
Target
SMT3
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Purity
Anti-Sumo Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Rabbit Anti-Sumo antibody reacts yeast SUMO tested by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended. A 12 kDa band corresponding to yeast SUMO is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. For ELISA a 1:1,000 to 1:5,000 dilution is recommended. Researchers should determine optimal titers for other applications.
Tested Applications
ELISA, WB
NCBI Accession Number
6320718
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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