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NFKB1 Antibody

CAT: 0013-GTR18187456Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187456Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NFKB1 antibody
Product Name Alternative
Rabbit Anti-NFKB p50 antibody, rabbit anti-NFKB1 antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
UniProt
P19838
Reactivity
Human
Immunogen
Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .
Target
NFKB1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
80 mg/ml
Dilution
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,000
Purity
This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-Human NFkB p50 (NFKB1) may react non-specifically with other proteins. Control peptide (code #100-4164p) will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This product was tested by immunoblot and ELISA. Anti-Human NFkB p50 (NFKB1) is suitable for the detection by immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105, and was found to be reactive against Human NFkB p50 (NFKB1) at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . No reaction was observed against the analogous mouse protein. This product was also tested in a gel supershift assay and found to be reactive against p50:p50 homodimers and p:50:p65 heterodimers using 0.5 to 1.0 μl per assay.
Tested Applications
ELISA, WB
NCBI Accession Number
CAB94757.1
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum

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