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SIRT1 Antibody

CAT: 0013-GTR18187061Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187061Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SIRT1 antibody
Product Name Alternative
Rabbit anti-SIRT1 antibody, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SirtT1 75 kDa fragment, 75SirT1, SIRT-1, SIRT 1, SIR2L1
UniProt
Q96EB6
Reactivity
Human
Immunogen
Affinity purified Anti-SIRT1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminal of human SIRT1 protein.
Target
SIRT1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1.07 mg/ml
Dilution
ELISA: 5.0 μg/ml, WB: 1.0 ug/ml
Purity
Anti-SIRT1 is directed against human SIRT1. This product is an affinity purified antibody produced by immunoaffinity chromatography. A BLAST analysis used to suggest reactivity with this protein from human, mouse, rat based on 100% homology for the immunizing sequence.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~62 or 81.7 kDa in size corresponding to SIRT1 by western blotting in the appropriate cell lysate or extract. Higher observed band ~125 kda is due to complexing proteins.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_001135970.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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