COL2A1 Antibody (Biotin)

CAT: 0013-GTR18184588Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18184588Size:100 µg

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Description

CO1A2 antibody

Product Name Alternative

Rabbit anti-Collagen Type II antibody biotin conjugation, biotin conjugated rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody

UniProt

P02458

Reactivity

Bovine, Human

Immunogen

Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage.

Target

COL2A1

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:5,000 - 1:10,000

Purity

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent) . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Notes

For research use only.

Applications Notes

Anti-Collagen Type II antibody is suitable for western blotting, IP, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

ELISA, IHC, IP, WB

NCBI Accession Number

NP_001835.3

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P02458

Collagen alpha-1(II) chain

CO2A1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P02458
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: COL2A1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: A6NGA0, Q12985, Q14009, Q14044, Q14045, Q14046, Q14047, Q14056, Q14058, Q16672, Q1JQ82, Q2V4X7, Q6LBY1, Q6LBY2, Q6LBY3, Q96IT5, Q99227, Q9UE38, Q9UE39, Q9UE40, Q9UE41, Q9UE42, Q9UE43

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-binding

Disease

CataractDeafnessDisease variantDwarfismStickler syndrome

Domain

CollagenRepeatSignal

PTM

Disulfide bondGlycoproteinHydroxylation

Cellular component

Extracellular matrixSecreted

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