COL3A1 Antibody (HRP)

CAT: 0013-GTR18184573Size: 50 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18184573Size:50 µg

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Description

Collagen Type III antibody (Peroxidase)

Product Name Alternative

Rabbit anti-Collagen Type III antibody peroxidase conjugation, HRP conjugated rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain

UniProt

P02461

Reactivity

Bovine, Human

Immunogen

Collagen Type III from human and bovine placenta

Target

COL3A1

Clonality

Polyclonal

Conjugation

HRP

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:1,000 - 1:5,000

Purity

This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Collagen Type III Peroxidase Conjugated Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

ELISA, IHC, IP, WB

NCBI Accession Number

NP_000081.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Thimerosal. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P02461

Collagen alpha-1(III) chain

CO3A1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P02461
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: COL3A1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: D2JYH5, D3DPH4, P78429, Q15112, Q16403, Q53S91, Q541P8, Q6LDB3, Q6LDJ2, Q6LDJ3, Q7KZ56, Q8N6U4, Q9UC88, Q9UC89, Q9UC90, Q9UC91, R4N3C5, V9GZI1

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Disease

Aortic aneurysmDisease variantEhlers-Danlos syndrome

Ligand

CalciumMetal-binding

Domain

CollagenRepeatSignal

PTM

Disulfide bondGlycoproteinHydroxylation

Cellular component

Extracellular matrixSecreted

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