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MCherry Antibody Pre-Adsorbed

CAT: 0013-GTR18184554Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184554Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MCherry antibody
Product Name Alternative
Rabbit anti-mCherry antibody, RFP, mCherry monomeric red fluorescent protein, Red Fluorescent Protein (RFP), rDsRed, Discosoma sp. Red Fluorescent Protein, mRFP1, mBanana, mHoneydew, mPlum, mOrange, mStrawberry, mTangerine
Reactivity
Other
Immunogen
The immunogen is a mCherry mutant variant fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Target
DsRed
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.17 mg/mL
Dilution
ELISA: 1:150,000 - 1:250,000, IHC: 1:200 - 1:2,000, IF: 1:200 - 1:2,000, WB: 1:2,000 - 1:10,000
Purity
MCherry was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified mCherry. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Form
Liquid (sterile filtered)
Storage Conditions
Store mCherry Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Polyclonal anti-mCherry is designed to detect mCherry, RFP, and its variants. Anti-mCherry (Discosoma sp.) has been tested by ELISA and Western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS) . Researchers should determine optimal titers for applications that are not stated. In addition, we performed conjugation of RFP antibodies to either fluorescent dyes, biotin or horseradish peroxidase to further facilitate RFP protein detection and quantification.
Tested Applications
ELISA, IF, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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