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POU5F1 Antibody

CAT: 0013-GTR18184487Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184487Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
42647 antibody
Product Name Alternative
Rabbit anti-OCT4 antibody, OCT-4, OCT 4, Octamer binding protein 4 antibody, Octamer binding transcription factor 3 antibody, OTF3 antibody, OCT3, POU5F1, POU class 5 homeobox 1 antibody, POU domain class 5 transcription factor 1 antibody
UniProt
Q01860
Reactivity
Human, Mouse
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.
Target
POU5F1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
0.59 mg/mL
Dilution
ELISA: 1:350,000, WB: 1:1,000 - 1:1,500
Purity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human and mouse Oct-4 protein. Cross-reactivity with Oct-4 from other species has not been tested. A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and western blotting.
Tested Applications
ELISA, WB
NCBI Accession Number
116235491
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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