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NES Antibody

CAT: 0013-GTR18184429Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184429Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nestin antibody
Product Name Alternative
Rabbit anti-Nestin antibody, rabbit anti-NES antibody, Nbla00170
UniProt
P48681
Reactivity
Human, Mouse
Immunogen
Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1484-1500 of human Nestin protein.
Target
NES
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Concentration
1.23 mg/mL
Dilution
ELISA: 1:20,000 - 1:80,000, IHC: 5ug/ml, WB: 1:500 - 1:3,000
Purity
This affinity purified antibody is directed against human Nestin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and Mesocricetus auratus (100% homology) . Based on protein sequence homology also expect partial reactivity against Nestin homologues from mouse (93%) and rat (88%) . Reactivity against homologues from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store anti-Nestin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Affinity purified Anti-Nestin has been tested for use in ELISA, Immunohistochemistry (IHC), and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
38176300
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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