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IL17a Antibody

CAT: 0013-GTR18184298Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184298Size:25 µL
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Description
IL-17A antibody
Product Name Alternative
Rat anti-IL-17A antibody, rat anti-interleukin17A antibody, Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8
UniProt
Q62386
Reactivity
Mouse
Immunogen
This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.
Target
IL17a
Clonality
Monoclonal
Clone
20B4.G10.F5
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
2.94 mg/mL
Dilution
ELISA: 1:10,000, FC: 1:500, WB: 1:1000
Purity
Anti-IL-17A is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse Il-17a protein. Cross-reactivity with Il-17a from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
IL-17A monoclonal antibody has been tested for use in western blotting, flow cytometry and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to the mature mouse Il17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.
Tested Applications
ELISA, FC, Multiplex Assay, WB
NCBI Accession Number
NP_034682.1
Host or Source
Rat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2a

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