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IL7 Antibody (HRP)

CAT: 0013-GTR18184242Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184242Size:100 µg
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Description
IL-7 antibody (Peroxidase)
Product Name Alternative
Rabbit anti-IL-7 peroxidase conjugated antibody, rabbit anti-interleukin-7 antibody, IL7, IL-7 Interleukin 7, Interleukin-7
UniProt
P13232
Reactivity
Human
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
Target
IL7
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested in western blotting and ELISA. By western blot a band approximately 17.4 kDa in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
DOT, ELISA, IHC, WB
NCBI Accession Number
NP_000871.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P13232

Interleukin-7

IL7_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P13232
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL7
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-7
EC number
—
Processing
Precursor
Secondary accessions
A0N0L3, Q5FBY5, Q5FBY9
Protein keywords

Technical term

3D-structureReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

CytokineGrowth factor

Disease

Disease variant

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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