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AnsB Antibody (Biotin)

CAT: 0013-GTR18184069Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184069Size:100 µg
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Description
L-Asparaginase antibody (Biotin)
Product Name Alternative
Rabbit anti-L-Asparaginase Antibody biotin Conjugation, biotin Conjugated rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
UniProt
P00805
Reactivity
E. coli
Immunogen
Asparaginase [E. coli]
Target
AnsB
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:1,000, WB: 1:500
Purity
Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli]. Cross reactivity against Asparaginase from other sources may occur but have not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-L-Asparaginase Biotin Conjugated Antibody has been by ELISA, dot blot, and western blot. This product is assayed against 1.0 ug of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:3,200 to 1:12,800 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL
Tested Applications
DOT, ELISA, WB
NCBI Accession Number
P00805.2
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P00805

L-asparaginase 2

ASPG2_ECOLI · Escherichia coli (strain K12)

View on UniProt ↗
Primary accession
P00805
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ansB
Protein existence
1: Evidence at protein level
Organism
Escherichia coli (strain K12)
Taxonomy ID
83333
Alternative names
—
EC number
3.5.1.1
Processing
Precursor
Secondary accessions
Q2M9N6
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalReference proteome

PTM

Disulfide bond

Molecular function

Hydrolase

Cellular component

Periplasm

Domain

Signal

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