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AnsB Antibody

CAT: 0013-GTR18183910Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183910Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
L-Asparaginase antibody
Product Name Alternative
Rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
UniProt
P00805
Reactivity
E. coli
Immunogen
Asparaginase [E. coli]
Target
AnsB
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/ml
Dilution
ELISA: 1:5,000 - 1:25,000, IP: 1:100, WB: 1:500 - 1:3,000
Purity
Anti-L-Asparaginase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.
Form
Liquid (sterile filtered)
References & Citations
José Gabriel da Silva Gomes 1, Larisse Cadeira Brandão 2, Daniel Pascoalino Pinheiro 3, Larissa Queiroz Pontes 3, Rômulo Farias Carneiro 4, Bárbara Cibelle Soares Farias Quintela 3, Anna Carolina Machado Marinho 3, Gilvan Pessoa Furtado 3, Bruno Anderson Matias Rocha Kinetics characterization of a low immunogenic recombinant l-asparaginase from Phaseolus vulgaris with cytotoxic activity against leukemia cells Int J Biol Macromol, (2024)
Pubmed id
38986978
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Anti-L-Asparaginase antibody has been tested by ELISA and western blot and is assayed in western blot and against 1.0 ug of Asparaginase [E. coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:400,000 is suggested for this product.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
P00805.2
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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