AAO Antibody (Biotin)

CAT: 0013-GTR18184043Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18184043Size:25 µL

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Description

Ascorbate Oxidase antibody (Biotin)

Product Name Alternative

Rabbit anti-Ascorbate Oxidase Antibody biotin Conjugation, biotin conjugated rabbit anti-Ascorbate Oxidase Antibody, L-ascorbate oxidase, Ascorbase, ASO

UniProt

P24792

Immunogen

Ascorbate Oxidase [cucurbita]

Target

AAO

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,500 - 1:15,000, IP: 1:100, WB: 1:500 - 1:5,000

Purity

Anti-Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidase from other sources may occur but have not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Ascorbate Oxidase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Ascorbate Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product

Tested Applications

ELISA, IP, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P24792

L-ascorbate oxidase

ASO_CUCMA · Cucurbita maxima

View on UniProt ↗

Primary accession: P24792
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: AAO
Protein existence: 1: Evidence at protein level
Organism: Cucurbita maxima (Pumpkin)
Taxonomy ID: 3661
Alternative names: ASO, Ascorbase
EC number: 1.10.3.3
Processing: Precursor
Secondary accessions: Q39539

Protein keywords

Ligand

CopperMetal-binding

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Molecular function

Oxidoreductase

Domain

RepeatSignal

Cellular component

Secreted

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