AAO Antibody (HRP)

CAT: 0013-GTR18183964Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18183964Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Ascorbate Oxidase antibody (Peroxidase)

Product Name Alternative

Rabbit anti-Ascorbate Oxidase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Ascorbate Oxidase Antibody, L-ascorbate oxidase, Ascorbase, ASO

UniProt

P24792

Immunogen

Ascorbate Oxidase [cucurbita]

Target

AAO

Clonality

Polyclonal

Conjugation

HRP

Concentration

1.0 mg/mL

Dilution

ELISA: 1:3,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:5,000

Purity

Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidase from other sources is unknown.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Ascorbate Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IP, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P24792

L-ascorbate oxidase

ASO_CUCMA · Cucurbita maxima

View on UniProt ↗

Primary accession: P24792
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: AAO
Protein existence: 1: Evidence at protein level
Organism: Cucurbita maxima (Pumpkin)
Taxonomy ID: 3661
Alternative names: ASO, Ascorbase
EC number: 1.10.3.3
Processing: Precursor
Secondary accessions: Q39539

Protein keywords

Ligand

CopperMetal-binding

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Molecular function

Oxidoreductase

Domain

RepeatSignal

Cellular component

Secreted

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