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PAP3 Antibody (Biotin)

CAT: 0013-GTR18184029Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184029Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Acid Phosphatase antibody (Biotin)
Product Name Alternative
Rabbit anti-Acid Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, PAP3, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody
UniProt
Q6J5M8
Reactivity
Plant
Immunogen
Acid Phosphatase [Potato]
Target
PAP3
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
WB: User Optimized
Purity
Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato]. Cross reactivity against Acid Phosphatase from other sources is unknown.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Acid Phosphatase Biotin Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Acid Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:32,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL
Tested Applications
DOT
NCBI Accession Number
AAT37528.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q6J5M8

Purple acid phosphatase

Q6J5M8_SOLTU · Solanum tuberosum

View on UniProt ↗
Primary accession
Q6J5M8
Review status
UniProtKB unreviewed (TrEMBL)
Gene
PAP3
Protein existence
2: Evidence at transcript level
Organism
Solanum tuberosum (Potato)
Taxonomy ID
4113
Alternative names
—
EC number
3.1.3.2
Processing
—
Secondary accessions
—
Protein keywords

PTM

Disulfide bondGlycoprotein

Molecular function

Hydrolase

Ligand

IronMetal-bindingZinc

Cellular component

Secreted

Domain

Signal

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