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XDH Antibody (HRP)

CAT: 0013-GTR18184005Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184005Size:100 µg
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Description
Xanthine Oxidase antibody (Peroxidase)
Product Name Alternative
Rabbit anti-Xanthine Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody
UniProt
P80457
Reactivity
Bovine
Immunogen
Xanthine Oxidase [Bovine Buttermilk]
Target
XDH
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Metabolism Research
Concentration
1.0 mg/mL
Dilution
IP: 1:100, WB: 1:250
Purity
Xanthine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk]. Cross reactivity against Xanthine Oxidase from other tissues and species may occur but have not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Xanthine Oxidase Peroxidase conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Xanthine Oxidase [Bovine Buttermilk] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:35,000 to 1:175,000 of the reconstitution concentration is suggested for this product.
Tested Applications
DOT, IP, WB
NCBI Accession Number
DAA24801.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P80457

Xanthine dehydrogenase/oxidase

XDH_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P80457
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
XDH
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q95325
Protein keywords

Ligand

2Fe-2SFADFlavoproteinIronIron-sulfurMetal-bindingMolybdenumNAD

Technical term

3D-structureDirect protein sequencingReference proteome

Cellular component

CytoplasmPeroxisomeSecreted

PTM

Disulfide bond

Molecular function

Oxidoreductase

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