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ALD2 Antibody (HRP)

CAT: 0013-GTR18183959Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183959Size:25 µL
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Description
Aldehyde Dehydrogenase antibody (Peroxidase)
Product Name Alternative
Rabbit anti-Aldehyde Dehydrogenase Antibody Peroxidase Conjugation, HRP conjugated anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase [NAD (P) +] 1, ALD2, ALD5, ALD
UniProt
P47771
Reactivity
Yeast
Immunogen
Aldehyde Dehydrogenase [Yeast]
Target
ALD2
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Purity
Aldehyde dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]. Cross reactivity against Aldehyde Dehydrogenase from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Aldehyde Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Aldehyde Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_013893.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P47771

Aldehyde dehydrogenase [NAD(P)+] 1

ALDH2_YEAST · Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

View on UniProt ↗
Primary accession
P47771
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ALD2
Protein existence
1: Evidence at protein level
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomy ID
559292
Alternative names
—
EC number
1.2.1.3
Processing
—
Secondary accessions
D6VZZ2
Protein keywords

Cellular component

Cytoplasm

Ligand

NAD

Molecular function

Oxidoreductase

Biological process

Pantothenate biosynthesis

Technical term

Reference proteome

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