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ALD2 Antibody (HRP)

CAT: 0013-GTR18183958Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183958Size:100 µg
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Description
Aldehyde Dehydrogenase antibody (Peroxidase)
Product Name Alternative
Rabbit anti-Aldehyde Dehydrogenase Antibody Peroxidase Conjugation, HRP conjugated anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase [NAD (P) +] 1, ALD2, ALD5, ALD
UniProt
P47771
Reactivity
Yeast
Immunogen
Aldehyde Dehydrogenase [Yeast]
Target
ALD2
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Purity
Aldehyde dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]. Cross reactivity against Aldehyde Dehydrogenase from other sources is unknown.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Aldehyde Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Aldehyde Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_013893.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P47771

Aldehyde dehydrogenase [NAD(P)+] 1

ALDH2_YEAST · Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

View on UniProt ↗
Primary accession
P47771
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ALD2
Protein existence
1: Evidence at protein level
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomy ID
559292
Alternative names
—
EC number
1.2.1.3
Processing
—
Secondary accessions
D6VZZ2
Protein keywords

Cellular component

Cytoplasm

Ligand

NAD

Molecular function

Oxidoreductase

Biological process

Pantothenate biosynthesis

Technical term

Reference proteome

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