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LDHA Antibody

CAT: 0013-GTR18184035Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184035Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Lactate Dehydrogenase antibody
Product Name Alternative
Goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M
UniProt
P13491
Reactivity
Human, Rabbit
Immunogen
This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
Target
LDHA
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Concentration
1.0 mg/ml
Dilution
ELISA: 1:2,000 - 1:10,000, IF: 1:200, IP: 1:100, WB: 1:1,000 - 1:5,000
Purity
Anti-LACTATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
LACTATE DEHYDROGENASE antibody is suitable for use in ELISA, western blot, and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, IP, WB
NCBI Accession Number
126050
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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