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GlnA Antibody (HRP)

CAT: 0013-GTR18183532Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183532Size:100 µg
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Description
Glutamine Synthetase antibody (Peroxidase)
Product Name Alternative
Goat anti-Glutamine Synthetase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase
UniProt
Q79VE3
Reactivity
Bacteria
Immunogen
Glutamine Synthetase [native from brevibacterium species]
Target
GlnA
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,500
Purity
Glutamine Synthetase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Glutamine Synthetase has been assayed against 1.0 ug of Glutamine Synthetase [Microbial] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
CAG33988.1
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q79VE3

Glutamine synthetase

Q79VE3_CORGL · Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)

View on UniProt ↗
Primary accession
Q79VE3
Review status
UniProtKB unreviewed (TrEMBL)
Gene
—
Protein existence
3: Inferred from homology
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)
Taxonomy ID
196627
Alternative names
—
EC number
6.3.1.2
Processing
—
Secondary accessions
O32354
Protein keywords

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Cellular component

Cytoplasm

Molecular function

Ligase

PTM

Phosphoprotein

Technical term

Reference proteome

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