Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, Fc,Avitag™ (MALS verified)

Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, Fc,Avitag™ (MALS verified)

• Background: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
• Description: Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, Fc,Avitag (CD7-C82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 141 (Accession # F7A802-1).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 42.0 kDa. The protein migrates as 57-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gln 19 - Glu 141
• Host: HEK 293
• Target: CD47
• Conjugation: Biotin-labeled
• Tag: C-Fc & C-Avi
• Source: Cynomolgus / Rhesus macaque
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 0.1 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Cynomolgus SIRP alpha, Fc Tag (Cat. No. SIA-C5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, Fc,Avitag (Cat. No. CD7-C82F3) with a linear range of 0.2-5 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 42.0 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C