Biotinylated Cynomolgus / Rhesus macaque LRRC15 / LIB Protein, His, Avitag™

• Background:

  LRRC15 (Leucine-rich repeat-containing protein 15) is also known as LIB and hLib. LRRC15 is highly expressed in a variety of solid tumors. LRRC15 was expressed on stromal fibroblasts in many solid tumors (e.g., breast, head and neck, lung, pancreatic) as well as directly on a subset of cancer cells of mesenchymal origin (e.g., sarcoma, melanoma, glioblastoma) . LRRC15 expression was induced by TGFβ on activated fibroblasts (αSMA+) and on mesenchymal stem cells. These collective findings suggested LRRC15 as a novel CAF and mesenchymal marker with utility as a therapeutic target for the treatment of cancers with LRRC15-positive stromal desmoplasia or cancers of mesenchymal origin. ABBV-085 is a monomethyl auristatin E (MMAE) -containing antibody–drug conjugate (ADC) directed against LRRC15, and it demonstrated robust preclinical efficacy against LRRC15 stromal-positive/cancer-negative, and LRRC15 cancer-positive models as a monotherapy, or in combination with standard-of-care therapies.

• Description:

  Biotinylated Cynomolgus / Rhesus macaque LRRC15, His, Avitag (LR5-C82E7) is expressed from human 293 cells (HEK293) . It contains AA Tyr 22 - Gly 538 (Accession # A0A2K5UKB6-1) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 61.6 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  A0A2K5UKB6-1

• Expression Region:

  Tyr 22 - Gly 538

• Host:

  HEK293

• Target:

  LRRC15

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Cynomolgus / Rhesus macaque

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Biotinylated Cynomolgus / Rhesus macaque LRRC15, His,Avitag (Cat. No. LR5-C82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Samrotamab with a linear range of 0.1-2 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  61.6 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Cynomolgus / Rhesus macaque