Thymidine-15N2

• Description :

  Thymidine-15N2 is the 15N labeled Thymidine[1]. Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[2][3][4].

• Product Name Alternative :

  DThyd-15N2; NSC-15N2

• UNSPSC :

  12352005

• Target :

  DNA/RNA Synthesis; Endogenous Metabolite; Orthopoxvirus

• Type :

  Isotope-Labeled Compounds

• Related Pathways :

  Anti-infection; Cell Cycle/DNA Damage; Metabolic Enzyme/Protease

• Applications :

  Cancer-programmed cell death

• Field of Research :

  Cancer

• Assay Protocol :

  https://www.medchemexpress.com/thymidine-15n2.html

• Purity :

  99.0

• Solubility :

  10 mM in DMSO|DMSO : 50mg/mL (ultrasonic)

• Smiles :

  O=C1[15N](C=C(C([15NH]1)=O)C)[C@]2([H])O[C@@H]([C@H](C2)O)CO

• Molecular Formula :

  C10H14 15N2O5

• Molecular Weight :

  244.22

• References & Citations :

  [1]Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53 (2) :211-216.|[2]Chen G, et al. Cell Synchronization by Double Thymidine Block. Bio Protoc. 2018 Sep 5;8 (17) .; FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181 (4604) :274-5.|[3]FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181 (4604) :274-5.|[4]Izeradjene K, et al. Inhibition of thymidine synthesis by folate analogues induces a Fas-Fas ligand-independentdeletion of superantigen-reactive peripheral T cells. Int Immunol. 2001 Jan13 (1) :85-93.

• Shipping Conditions :

  Room Temperature

• Storage Conditions :

  4°C (Powder, sealed storage, away from moisture and light)

• Scientific Category :

  Isotope-Labeled Compounds

• Clinical Information :

  No Development Reported