GOT1 Antibody (HRP)

CAT: 0013-GTR18184123Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18184123Size:100 µg

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Description

Aspartate Transaminase antibody (Peroxidase)

Product Name Alternative

Sheep anti-Aspartate Transaminase HRP conjugated Antibody, peroxidase conjugated sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

UniProt

P00503

Reactivity

Porcine

Immunogen

Aspartate Aminotransferase (AST) [Pig Heart]

Target

GOT1

Clonality

Polyclonal

Conjugation

HRP

Concentration

1 mg/mL

Dilution

ELISA: 1:10,000 - 1:40,000, WB: 1:1,000 - 1:5,000

Purity

Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart]. Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Aspartate Transaminase Peroxidase Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 ug of Aspartate Aminotransferase (AST) [Pig Heart] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Tested Applications

DOT, ELISA, WB

NCBI Accession Number

NP_999092.1

Host or Source

Sheep

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P00503

Aspartate aminotransferase, cytoplasmic

AATC_PIG · Sus scrofa

View on UniProt ↗

Primary accession: P00503
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: GOT1
Protein existence: 1: Evidence at protein level
Organism: Sus scrofa (Pig)
Taxonomy ID: 9823
Alternative names: cAspAT
EC number: 2.6.1.1, 2.6.1.3
Processing: —
Secondary accessions: —

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Biological process

Amino-acid biosynthesis

Molecular function

AminotransferaseTransferase

Cellular component

Cytoplasm

Ligand

Pyridoxal phosphate

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