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VEGFA Antibody (HRP)

CAT: 0013-GTR18184245Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184245Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
VEGF antibody (Peroxidase)
Product Name Alternative
Rabbit anti-VEGF Peroxidase Conjugated antibody, rabbit anti-VEGF-165 Peroxidase Conjugated antibody, Vascular endothelial growth factor A, VEGF-A, VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor, VPF
UniProt
P15692
Reactivity
Human
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
Target
VEGFA
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Cardiovascular Research; Cell Biology
Concentration
1.0 mg/mL
Dilution
IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This protein-A purified antibody has been tested for use in western blotting. Reactivity in other assays has not been determined. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
IHC, WB
NCBI Accession Number
NP_001165097.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P15692

Vascular endothelial growth factor A, long form

VEGFA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15692
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VEGFA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
L-VEGF
EC number
—
Processing
Precursor
Secondary accessions
B5BU86, H0Y2S8, H0Y407, H0Y414, H0Y462, H0Y8N2, H3BLW7, O60720, O75875, Q074Z4, Q16889, Q5UB46, Q6P0P5, Q96KJ0, Q96L82, Q96NW5, Q9H1W8, Q9H1W9, Q9UH58, Q9UL23
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative initiationAlternative promoter usageAlternative splicing

Biological process

AngiogenesisDifferentiation

Cellular component

CytoplasmEndoplasmic reticulumExtracellular matrixGolgi apparatusNucleusSecreted

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

PTM

Disulfide bondGlycoprotein

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