EcoPlexTM Mouse Th1/Th2 8-Plex Panel Kit

CAT: 0763-MPM007Size: 96 TestsDry Ice: NoHazardous: No
CAT#:0763-MPM007Size:96 Tests
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
IFNγ, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-10, and TNFα
UniProt
IL-2:P04351 IL-1α:P01582 IFN-γ:P01580 IL-4:P07750 IL-6:P08505 IL-5:P04401 IL-10:P18893 TNF-α:P06804
Cross Reactivity
Cross-reactivity of analytes in the Panel: Negligible
Assay Principle
EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.
Assay Performance Time
3 h
Sample Type
Serum, plasma, bodily fluid, cell culture supernatant
Precision
Intra-assay CV<10% Inter-assay CV<20%
Recovery
70%-130%
Sensitivity
IL-2<2pg/mL IL-1α<2pg/mL IFN-γ<0.5pg/mL IL-4<0.5pg/mL IL-6<3pg/mL IL-5<5pg/mL IL-10<2pg/mL TNF-α<0.5pg/mL
Shipping Conditions
Ice bag
Storage Conditions
-20°C,12 months
Species
Mouse
Recommended Usage
This kit is for detection of IFN-γ, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-10 and TNF-α in mouse serum, plasma, biological fluid and cell culture supernatant.
Product Datasheet
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