GRK5 Antibody (C-term) [APR05833G]

• Background:

  Tissue Location: Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.

• Synonyms:

  G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5, GRK5, GPRK5

• Gene Name:

  GRK5

• Gene ID:

  2869

• UniProt:

  P34947

• Chromosomal Location:

  Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca (2+) /calmodulin-dependent fashion

• Applications:

  WB, IHC-P

• Dilution:

  WB--1:1000 IHC-P--1:100~500

• Form:

  Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

• Function:

  Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs) . Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro) .

• Molecular Weight:

  67787

• Storage Conditions:

  Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

• Overview:

  We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GRK5 Antibody (C-term) [APR05833G].

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/?term=2869

• Uniprot URL:

  https://www.uniprot.org/uniprot/P34947