Anti-Hu CD71 PerCP-Cy1535.5

Anti-Hu CD71 PerCP-Cy1535.5

• Background: CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
• Specifications: The antibody MEM-75 reacts with an extracellular epitope of CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: NALM-6 human pre-B cell line
• Target Antigen: CD71
• Isotype: IgG1
• Clone: MEM-75
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: PerCP-Cy1535.5
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Beck Z, Balogh A, Kis A, Izsépi E, Cervenak L, László G, Bíró A, Liliom K, Mocsár G, Vámosi G, Füst G, Matko J: New cholesterol-specific antibodies remodel HIV-1 target cells' surface and inhibit their in vitro virus production. J Lipid Res. 2010 Feb51(2):286-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/19654424,*Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2019 Nov 8. doi: 10.1002/cyto.b.21853. , URL: https://www.ncbi.nlm.nih.gov/pubmed/31705743,
• Other References: *Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 2113(35):4725-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/17729394?ordin,*Graham RM, Reutens GM, Herbison CE, Delima RD, Chua AC, Olynyk JK, Trinder D: Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron. J Hepatol. 2008 Feb48(2):327-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/18083267?ordin,*Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr205(4):297-318., URL: https://www.ncbi.nlm.nih.gov/pubmed/15750326?ordin,*Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec1(3):E79., URL: https://www.ncbi.nlm.nih.gov/pubmed/14691550?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.