Anti-Hu CD10 PerCP-Cy1535.5

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAT:

270-T9-209-T100

Size:

100 Tests

Price:

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For price, please contact [email protected].

Anti-Hu CD10 PerCP-Cy1535.5

Background: CD10 (neutral endopeptidase 8211 NEP, common acute lymphocytic leukemia antigen 8211 CALLA, membrane metallo-endopeptidase 8211 MME, enkefalinase) is a 100-kDa cell surface zinc metalloprotease, cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.
Specifications: The antibody MEM-78 reacts with an extracellular epitope CD10 antigen (CALLA - Common acute lymphatic leukemia antigen), a 100 kDa type II integral membrane protein.
Certification: RUO
Host: Mouse
Species Reactivity: Human
Immunogen: NALM-6 human pre-B cell line
Target Antigen: CD10
Isotype: IgG1
Clone: MEM-78
Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
Validated Applications: FC
Format: PerCP-Cy1535.5
Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations: *Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 198834(1):23-34. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2968928?dopt=A,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL: ,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. Blood. 1999 Feb 1593(4):1437-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10075459?itool,
Other References: *Suzuki T, Ino K, Kikkawa F, Uehara C, Kajiyama H, Shibata K, Mizutani S: Neutral endopeptidase/CD10 expression during phorbol ester-induced differentiation of choriocarcinoma cells through the protein kinase C- and extracellular signal-regulated kinase-dependent signalling pathway. Placenta. 2002 Jul23(6):475-82., URL: https://pubmed.ncbi.nlm.nih.gov/12137745/,*Yada K, Kashima K, Daa T, Kitano S, Fujiwara S, Yokoyama S: Expression of CD10 in basal cell carcinoma. Am J Dermatopathol. 2004 Dec26(6):463-71., URL: https://pubmed.ncbi.nlm.nih.gov/15618927/,*Braham H, Trimeche M, Ziadi S, Mestiri S, Mokni M, Amara K, Hachana M, Sriha B, Korbi S: CD10 expression by fusiform stromal cells in nasopharyngeal carcinoma correlates with tumor progression. Virchows Arch. 2006 Aug449(2):220-4., URL: https://pubmed.ncbi.nlm.nih.gov/16673118/,*Dall'Era MA, True LD, Siegel AF, Porter MP, Sherertz TM, Liu AY: Differential expression of CD10 in prostate cancer and its clinical implication. BMC Urol. 2007 Mar 27:3., URL: https://pubmed.ncbi.nlm.nih.gov/17335564/,
Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

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