Anti-Hu CD15 PE-Cy1535

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

Alternative Products

Cat	Name
MBS1801060-01	Anti-Hu CD15 PE
MBS1801060-02	Anti-Hu CD15 PE
MBS1801342-01	Anti-Hu CD15 PE
MBS1801342-02	Anti-Hu CD15 PE
MBS1801343-01	Anti-Hu CD15 PE-Cyanine5
MBS1801343-02	Anti-Hu CD15 PE-Cyanine5
MBS1801058-01	Anti-Hu CD15 Biotin
MBS1801057-01	Anti-Hu CD15 APC
MBS1801340-02	Anti-Hu CD15 Purified
MBS1801056-01	Anti-Hu CD15 Purified

CAT:

270-T8-138-T100

Size:

100 Tests

Price:

Ask

For price, please contact [email protected].

Anti-Hu CD15 PE-Cy1535

Background: CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Specifications: The mouse monoclonal antibody MMA reacts with an extracellular epitope of CD15, a cell membrane 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells it is also present on Langerhans cells and some myeloid precursors cells. This antibody is a superior reagent for identifying of Hodgkin´s lymphoma.
Certification: RUO
Host: Mouse
Species Reactivity: Human
Immunogen: U937 histiocytic lymphoma cells
Target Antigen: CD15
Isotype: IgM κ
Clone: MMA
Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
Validated Applications: FC
Format: PE-Cy1535
Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations: *Chaubey S, Wolfe JH: Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage. Stem Cells Transl Med. 2013 Jun2(6):444-54., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=chaubey+s+wolfe+jh ,*Mao XG, Zhang X, Xue XY, Guo G, Wang P, Zhang W, Fei Z, Zhen HN, You SW, Yang H: Brain Tumor Stem-Like Cells Identified by Neural Stem Cell Marker CD15. Transl Oncol. 2009 Dec2(4):247-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/19956386 ,*Capela A, Temple S: LeX is expressed by principle progenitor cells in the embryonic nervous system, is secreted into their environment and binds Wnt-1. Dev Biol. 2006 Mar 15291(2):300-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/16458284 ,*Pellegrini W, Bresciani G, De Zorzi A, Marocolo D, Ungari M, Facchetti F: MMA monoclonal antibody is a superior anti-CD15 reagent for the diagnosis of classical Hodgkin's lymphoma? Haematologica. 2007 May92(5):708-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=pellegrini+w+bresciani+g,*Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan94(1):23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353,*Sutherland DR, Ortiz F, Quest G, Illingworth A, Benko M, Nayyar R, Marinov I: High-sensitivity 5-, 6-, and 7-color PNH WBC assays for both Canto II and Navios platforms. Cytometry B Clin Cytom. 2018 Jul94(4):637-651., URL: https://www.ncbi.nlm.nih.gov/pubmed/29381839 ,
Other References: *Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep39(1-2):185-94., URL: https://pubmed.ncbi.nlm.nih.gov/10975398/,*Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov24(11):771-6., URL: https://pubmed.ncbi.nlm.nih.gov/1362193/,*Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1406(2):333-41., URL: https://pubmed.ncbi.nlm.nih.gov/17497959/,
Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Complementary Product Range

Our range of products, including Anti-Hu CD15 PE-Cy1535, falls within the same field of application and can be used across various research projects. These products are carefully selected for their high performance and reliability, supporting the diverse needs of modern laboratories. Whether for ongoing studies or future experiments, they are designed to help researchers achieve their scientific goals. Here are some related products that may be of interest for similar or future applications:

Each item in our collection, is tested for precision and quality, ensuring dependable solutions for your research. As we continue to incorporate the latest advancements, Gentaur ensures that our product range remains relevant and versatile, providing you with the tools needed for both current and future projects.

Find us on

Cat	Name
T8-138-T100	Anti-Hu CD15 PE-Cy1535
T8-146-T100	Anti-Hu CD184 PE-Cy1535
T8-183-T100	Anti-Hu CD7 PE-Cy1535
T8-207-T025	Anti-Hu CD8 PE-Cy1535
T8-209-T025	Anti-Hu CD10 PE-Cy1535
T8-231-T100	Anti-Hu CD56 PE-Cy1535
T8-273-T025	Anti-Hu CD31 PE-Cy1535
T8-308-T100	Anti-Hu CD27 PE-Cy1535
T8-320-C100	Anti-Hu IgM PE-Cy1535
T8-343-T025	Anti-Hu CD63 PE-Cy1535