Anti-Hu CD7 PE-DyLight™ 594

• Description:

  CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly) . CD7 is also expressed on acute lymphocytic leukemia (ALL) . CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  Human acute myelogenous leukaemia cell line KG-1.

• Target Antigen:

  CD7

• Clone:

  MEM-186

• Applications:

  FC (QC tested)

• Format:

  PE-DyLight™ 594

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Weijler L, Kowarsch F, Wödlinger M, Reiter M, Maurer-Granofszky M, Schumich A, Dworzak MN: UMAP based anomaly detection for minimal residual disease quantification within acute myeloid leukemia. Cancers (Basel) 2022 Feb 11;14 (4) :898., URL: https://pubmed.ncbi.nlm.nih.gov/35205645/

• Other References:

  *Nishimura M, Takanashi M, Okazaki H, Satake M, Nakajima K: Role of CD7 expressed in lung microvascular endothelial cells as Fc receptor for immunoglobulin M. Endothelium. 2006 Jul-Aug;13 (4) :287-92., URL: https://pubmed.ncbi.nlm.nih.gov/16990185/, *Chang H, Yeung J, Brandwein J, Yi QL: CD7 expression predicts poor disease free survival and post-remission survival in patients with acute myeloid leukemia and normal karyotype. Leuk Res. 2007 Feb;31 (2) :157-62. Epub 2006 Jul 11., URL: https://pubmed.ncbi.nlm.nih.gov/16837044/, *Lam GK, Liao HX, Xue Y, Alam SM, Scearce RM, Kaufman RE, Sempowski GD, Haynes BF. Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma. J Clin Immunol. 2005 Jan;25 (1) :41-9., URL: https://pubmed.ncbi.nlm.nih.gov/15742156/, *Alaibac M, Pigozzi B, Belloni-Fortina A, Michelotto A, Saponeri A, Peserico A. CD7 expression in reactive and malignant human skin T-lymphocytes. Anticancer Res. 2003 May-Jun;23 (3B) :2707-10., URL: https://pubmed.ncbi.nlm.nih.gov/12894562/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody MEM-186 reacts with an extracellular epitope of CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.

• Applications Notes:

  6

• Isotype:

  Mouse IgG1 kappa