Anti-Hu CD49b PE-DyLight™ 594

• Description:

  CD49b the integrin alpha 2 chain, associates with CD29 (integrin beta 1 chain) to form VLA-2 integrin complex, which plays a critical role in the processes of lymphocyte adhesion and activation. VLA-2 serves as a receptor for collagen, laminin, and fibronectin and also regulates the extracellular matrix synthesis and organization. CD49b has been used to identify NK cells, and coexpressed with CD223 (LAG-3) it identifies CD4+ T regulatory type 1 cells (Tr1) .

• Certification:

  RUO

• Reactivity:

  Non-human primates, Human

• Immunogen:

  Human platelets

• Target Antigen:

  CD49b

• Clone:

  AK7

• Applications:

  FC (QC tested)

• Format:

  PE-DyLight™ 594

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Müller K, Schinn M, Reichrath J, Meineke V: 1alpha,25-Dihydroxyvitamin D3 modulates the response of human keratinocytes to ionizing radiation exposure. Anticancer Res. 2006 Jul-Aug;26 (4A) :2735-41. , URL: http://www.ncbi.nlm.nih.gov/pubmed/16886685 , *Engl T, Makarević J, Relja B, Natsheh I, Müller I, Beecken WD, Jonas D, Blaheta RA: Mycophenolate mofetil modulates adhesion receptors of the beta1 integrin family on tumor cells: impact on tumor recurrence and malignancy. BMC Cancer. 2005 Jan 11;5:4., URL: http://www.ncbi.nlm.nih.gov/pubmed/15644133 , *Sutavani RV, Bradley RG, Ramage JM, Jackson AM, Durrant LG, Spendlove I: CD55 costimulation induces differentiation of a discrete T regulatory type 1 cell population with a stable phenotype. J Immunol. 2013 Dec 15;191 (12) :5895-903., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=sutavani+rv+bradley+rg+ramage+jm , *Gamble JR, Matthias LJ, Meyer G, Kaur P, Russ G, Faull R, Berndt MC, Vadas MA: Regulation of in vitro capillary tube formation by anti-integrin antibodies. J Cell Biol. 1993 May;121 (4) :931-43., URL: http://www.ncbi.nlm.nih.gov/pubmed/8491784, *Londrigan SL, Hewish MJ, Thomson MJ, Sanders GM, Mustafa H, Coulson BS: Growth of rotaviruses in continuous human and monkey cell lines that vary in their expression of integrins. J Gen Virol. 2000 Sep;81 (Pt 9) :2203-13., URL: https://pubmed.ncbi.nlm.nih.gov/10950978/, *Werr J, Johansson J, Eriksson EE, Hedqvist P, Ruoslahti E, Lindbom L: Integrin alpha (2) beta (1) (VLA-2) is a principal receptor used by neutrophils for locomotion in extravascular tissue. Blood. 2000 Mar 1;95 (5) :1804-9., URL: https://pubmed.ncbi.nlm.nih.gov/10688841/, *Ammon C, Meyer SP, Schwarzfischer L, Krause SW, Andreesen R, Kreutz M: Comparative analysis of integrin expression on monocyte-derived macrophages and monocyte-derived dendritic cells. Immunology. 2000 Jul;100 (3) :364-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=ammon+c+m, *Ciarlet M, Crawford SE, Cheng E, Blutt SE, Rice DA, Bergelson JM, Estes MK: VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment. J Virol. 2002 Feb;76 (3) :1109-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/11773387 , *Kögler G, Sensken S, Airey JA, Trapp T, Müschen M, Feldhahn N, Liedtke S, Sorg RV, Fischer J, Rosenbaum C, Greschat S, Knipper A, Bender J, Degistirici O, Gao J, Caplan AI, Colletti EJ, Almeida-Porada G, Müller HW, Zanjani E, Wernet P: A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med. 2004 Jul 19;200 (2) :123-35., URL: https://pubmed.ncbi.nlm.nih.gov/15263023/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody AK7 recognizes an extracellular epitope of CD49b, a 160-165 kDa alpha subunit of VLA-2 integrin complex expressed on platelets, megakaryocytes, activated T and B cells, monocytes, epithelial cells, endothelial cells and fibroblasts.

• Applications Notes:

  6

• Isotype:

  Mouse IgG1