Anti-Hu CD45 Pacific Orange153

Anti-Hu CD45 Pacific Orange153

• Background: CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
• Specifications: The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Non-human primates
• Immunogen: human peripheral blood leukocytes
• Target Antigen: CD45
• Isotype: Igg1 κ
• Clone: HI30
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: Pacific Orange153
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Tembhare PR, Subramanian PG, Sehgal K, Yajamanam B, Kumar A, Gadge V, Inamdar N, Gujral S: Immunophenotypic profile of plasma cell leukemia: a retrospective study in a reference cancer center in India and review of literature. Indian J Pathol Microbiol. 2011 Apr-Jun54(2):294-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/21623077,*Cubillos-Ruiz JR, Martinez D, Scarlett UK, Rutkowski MR, Nesbeth YC, Camposeco-Jacobs AL, Conejo-Garcia JR: CD277 is a negative co-stimulatory molecule universally expressed by ovarian cancer microenvironmental cells. Oncotarget. 2010 Sep1(5):329-38., URL: https://www.ncbi.nlm.nih.gov/pubmed/21113407,*Song L, Ara T, Wu HW, Woo CW, Reynolds CP, Seeger RC, DeClerck YA, Thiele CJ, Sposto R, Metelitsa LS: Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma. J Clin Invest. 2007 Sep117(9):2702-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/17710228,*Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function. Eur Heart J. 2005 Jun26(12):1196-204., URL: https://www.ncbi.nlm.nih.gov/pubmed/15734770,*Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW Jr, Arthur LO, Ott DE, Lifson JD: Differential incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and major histocompatibility complex class I and II molecules into human immunodeficiency virus type 1 virions and microvesicles: implications for viral pathogenesis and immune regulation. J Virol. 2001 Jul75(13):6173-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/11390619,*Yamada T, Zhu D, Saxon A, Zhang K: CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase. J Biol Chem. 2002 Aug 9277(32):28830-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/11994288,*Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE: A novel marker of human endometrial mesenchymal stem-like cells. Cell Transplant. 201221(10):2201-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/22469435,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,
• Other References: *Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15105(8):3295-302. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15626731?ordin,*Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct16(4):256-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/17107594?ordina,*Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15176(6):3417-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/16517710?ordina,*Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul44(15):3682-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/17524482?ordina,*Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19. , URL: http://www.ncbi.nlm.nih.gov/pubmed/18157742?ordina,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.