Anti-Hu CD45 Pacific Orange153

Anti-Hu CD45 Pacific Orange153

• Background: CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
• Specifications: The mouse monoclonal antibody 2D1 reacts with an extracellular epitope of all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human peripheral blood mononuclear cells
• Target Antigen: CD45
• Isotype: Igg1 κ
• Clone: 2D1
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: Pacific Orange153
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Wellhausen SR, Slone SP, Miller JJ: Clone-specific anti-CD45 blocking factor in patient plasma. Cytometry B Clin Cytom. 2007 Sep72(5):423-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/17311353,*Janossy G, Jani IV, Bradley NJ, Bikoue A, Pitfield T, Glencross DK: Affordable CD4(+)-T-cell counting by flow cytometry: CD45 gating for volumetric analysis. Clin Diagn Lab Immunol. 2002 Sep9(5):1085-94., URL: https://www.ncbi.nlm.nih.gov/pubmed/12204964,*Ito A, Ishida T, Yano H, Inagaki A, Suzuki S, Sato F, Takino H, Mori F, Ri M, Kusumoto S, Komatsu H, Iida S, Inagaki H, Ueda R: Defucosylated anti-CCR4 monoclonal antibody exercises potent ADCC-mediated antitumor effect in the novel tumor-bearing humanized NOD/Shi-scid, IL-2Rgamma(null) mouse model. Cancer Immunol Immunother. 2009 Aug58(8):1195-206., URL: https://pubmed.ncbi.nlm.nih.gov/19048251/,*Pfau JC, Walker E, Card GL: Monoclonal antibodies to CD45 modify LPS-induced arachidonic acid metabolism in macrophages. Biochim Biophys Acta. 2000 Feb 281495(3):212-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/10699460,*Mariucci S, Rovati B, Bencardino K, Manzoni M, Danova M: Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects. Int J Lab Hematol. 2010 Feb32(1 Pt 1):e40-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/20088999,*Vicetti Miguel RD, Harvey SA, LaFramboise WA, Reighard SD, Matthews DB, Cherpes TL: Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity. PLoS One. 20138(3):e58565., URL: https://www.ncbi.nlm.nih.gov/pubmed/23555586,*Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan94(1):23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353,
• Other References: *Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15105(8):3295-302. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15626731?ordin,*Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct16(4):256-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/17107594?ordina,*Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15176(6):3417-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/16517710?ordina,*Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul44(15):3682-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/17524482?ordina,*Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19. , URL: http://www.ncbi.nlm.nih.gov/pubmed/18157742?ordina,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.