Anti-Phosphotyrosine Alexa Fluor® 647

Anti-Phosphotyrosine Alexa Fluor® 647

• Specifications: The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Species independent
• Immunogen: Phosphotyrosine conjugated to bovine serum albumin.
• Target Antigen: Phosphotyrosine
• Isotype: IgG1
• Clone: P-Tyr-01
• Applications: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
• Validated Applications: WB, ICC, FC
• Concentration: 1 mg/mL
• Format: Alexa Fluor® 647
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 94:e10766., URL: https://www.ncbi.nlm.nih.gov/pubmed/26650353 ,*Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16196(12):1617-26., URL: http://www.ncbi.nlm.nih.gov/pubmed/12486104?itool=,*Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15178(4):2262-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/17277131?ordin,*Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1191(9):1591-604., URL: https://pubmed.ncbi.nlm.nih.gov/10790433/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.