Anti-Hu IgG (Fc) HRP

• Description:

  Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.

• Certification:

  RUO

• Immunogen:

  Fusion protein of human IgG Fc fragment.

• Target Antigen:

  IgG (Fc)

• Clone:

  EM-07

• Applications:

  WB, ELISA

• Concentration:

  0.2 mg/mL

• Format:

  HRP

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal

• Other References:

  *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh) . 1975;194:77-95. , URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp, *Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21 (6) :667-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/1122610?ordina, *Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39 (2) :129-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/6966908?ordina, *Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152 (2) :323-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/8258141?ordina

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.

• Applications Notes:

  Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1