Anti-Perforin PE

Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9) . It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.

Human, Cow

Purified granules from human YT lymphoma cell line

Perforin

DG9

FC-IC (QC tested)

PE

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α (-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134 (3) :326-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/21978002, *Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2 (3) :139-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/20348186 , *Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8 (11) :e1002984., URL: http://www.ncbi.nlm.nih.gov/pubmed/23144609 , *Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73 (11) :1050-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18615597 , *Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140 (5) :1025-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/1374586

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

6